Overview

  • Product name
  • Description
    Rabbit polyclonal to Mitoferrin1
  • Tested applications
    Suitable for: ICC, ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Recombinant fragment
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of mouse Mitoferrin-1

  • General notes

    This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab56134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/200.
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Mitoferrin1, SLC25A37, belongs to the mitochondrial carrier family and contains 3 Solcar repeats. It is a mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells and plays an essential role in heme biosynthesis.
  • Cellular localization
    Mitochondrial inner membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • HT015 antibody
    • MFRN antibody
    • Mitochondrial iron transporter 1 antibody
    • Mitochondrial solute carrier protein antibody
    • Mitoferrin 1 antibody
    • MSC antibody
    • MSCP antibody
    • predicted protein of HQ2217 antibody
    • PRO1278 antibody
    • PRO1584 antibody
    • PRO2217 antibody
    • Solute carrier family 25 member 37 antibody
    see all

Images

  • ab56134 staining cultured rat iron treated astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde in 0.3% TritonX in 0.1% PBS for 5 minutes and blocked with 10% donkey serum in 0.3% TritonX in 0.1% PBS for 30 minutes at 24°C. The cultured cells were then stained with ab561340 at 1/200 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 24h at 4°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Hoechst was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

References

ab56134 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (DRG neurons)
Specification
DRG neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (Iron treated astrocytes)
Specification
Iron treated astrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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