Overview

  • Product nameAnti-Mitoferrin1 antibody
    See all Mitoferrin1 primary antibodies
  • Description
    Rabbit polyclonal to Mitoferrin1
  • Tested applicationsSuitable for: ICC, ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Recombinant Fragment
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of mouse Mitoferrin-1

Properties

Applications

Our Abpromise guarantee covers the use of ab56134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/200.
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceMitoferrin1, SLC25A37, belongs to the mitochondrial carrier family and contains 3 Solcar repeats. It is a mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells and plays an essential role in heme biosynthesis.
  • Cellular localizationMitochondrial inner membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • HT015 antibody
    • MFRN antibody
    • Mitochondrial iron transporter 1 antibody
    • Mitochondrial solute carrier protein antibody
    • Mitoferrin 1 antibody
    • MSC antibody
    • MSCP antibody
    • predicted protein of HQ2217 antibody
    • PRO1278 antibody
    • PRO1584 antibody
    • PRO2217 antibody
    • Solute carrier family 25 member 37 antibody
    see all

Anti-Mitoferrin1 antibody images

  • ab56134 staining cultured rat iron treated astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde in 0.3% TritonX in 0.1% PBS for 5 minutes and blocked with 10% donkey serum in 0.3% TritonX in 0.1% PBS for 30 minutes at 24°C. The cultured cells were then stained with ab561340 at 1/200 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 24h at 4°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Hoechst was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

References for Anti-Mitoferrin1 antibody (ab56134)

ab56134 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (DRG neurons)
Specification DRG neurons
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cultured Cells (Iron treated astrocytes)
Specification Iron treated astrocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"