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Product name Anti-Mitofilin antibody [2E4AD5] See all Mitofilin primary antibodies
Mouse monoclonal [2E4AD5] to Mitofilin
Tested applications Suitable for:
WB, ICC/IF, IHC-P, Flow Cyt, In-Cell ELISA, IP more details
Mouse, Rat, Cow, Human
Human heart mitochondria.
Human fibroblasts; Human cerebellum tissue; Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; HL60 cells.
Product was previously marketed under the MitoSciences sub-brand.
Storage instructions Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer Preservative: 0.02% Sodium azide Constituent: HBS
Concentration information loading...
Purity >95% by SDS-PAGE
Purification notes ab110329 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clone number 2E4AD5
Light chain type kappa
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Predicted molecular weight: 84 kDa.
Use a concentration of 1 - 5 µg/ml.
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use a concentration of 4 µg/ml.
Use at an assay dependent concentration.
Cellular localization Mitochondrion inner membrane.
Information by UniProt
Cell proliferation inducing protein 52 antibody
Cell proliferation-inducing gene 4/52 protein antibody
Heart muscle protein antibody
Anti-Mitofilin antibody [2E4AD5] images
Immunocytochemistry/ Immunofluorescence - Mitofilin antibody [2E4AD5 ] (ab110329)
Immunocytochemistry analysis using ab110329 at 5µg/ml staining Mitofilin in Cultured Human fibroblasts. (Fixed and permeabilized) followed by a fluorescent goat-anti-mouse IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mitofilin antibody [2E4AD5 ] (ab110329)
Immunohistological analysis using ab110329 at 1/100 dilution staining Mitofilin in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
Western blot - Mitofilin antibody [2E4AD5 ] (ab110329)
All lanes : Anti-Mitofilin antibody [2E4AD5] (ab110329) at 2 µg/ml Lane 1 : Isolated mitochondria from Human heart at 5 µg Lane 2 : Isolated mitochondria from Bovine heart at 1 µg Lane 3 : Isolated mitochondria from Rat heart at 10 µg Lane 4 : Isolated mitochondria from Mouse heart at 10 µg Lane 5 : HepG2 cell lysate at 20 µg Predicted band size : 84 kDa
Flow Cytometry - Mitofilin antibody [2E4AD5 ] (ab110329)
Flow cytometric analysis using ab110329 at 1µg/ml staining Mitofilin in HL60 cells (blue). Isotype control antibody (red).
References for Anti-Mitofilin antibody [2E4AD5] (ab110329)
This product has been referenced in:
et al. ROMO1 is an essential redox-dependent regulator of mitochondrial dynamics. Sci Signal 7:ra10 (2014).
Read more (PubMed: 24473195) »
et al. Recovery of Indicators of Mitochondrial Biogenesis, Oxidative Stress, and Aging With (-)-Epicatechin in Senile Mice. J Gerontol A Biol Sci Med Sci N/A:N/A (2014).
Read more (PubMed: 25143004) »
See all 12 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"