Overview

  • Product nameAnti-Mitofilin antibody [2E4AD5]
    See all Mitofilin primary antibodies
  • Description
    Mouse monoclonal [2E4AD5] to Mitofilin
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-P, Flow Cyt, In-Cell ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Human heart mitochondria.

  • Positive control
    • Human fibroblasts; Human cerebellum tissue; Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; HL60 cells.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand. 

Properties

Applications

Our Abpromise guarantee covers the use of ab110329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 84 kDa.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
In-Cell ELISA Use a concentration of 4 µg/ml.
IP Use at an assay dependent concentration.

Target

Anti-Mitofilin antibody [2E4AD5] images

  • Immunocytochemistry analysis using ab110329 at 5µg/ml staining Mitofilin in Cultured Human fibroblasts. (Fixed and permeabilized) followed by a fluorescent goat-anti-mouse IgG.
  • Immunohistological analysis using ab110329 at 1/100 dilution staining Mitofilin in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
  • All lanes : Anti-Mitofilin antibody [2E4AD5] (ab110329) at 2 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : HepG2 cell lysate at 20 µg


    Predicted band size : 84 kDa
  • Flow cytometric analysis using ab110329 at 1µg/ml staining Mitofilin in HL60 cells (blue). Isotype control antibody (red).

References for Anti-Mitofilin antibody [2E4AD5] (ab110329)

This product has been referenced in:
  • Norton M  et al. ROMO1 is an essential redox-dependent regulator of mitochondrial dynamics. Sci Signal 7:ra10 (2014). Read more (PubMed: 24473195) »
  • Moreno-Ulloa A  et al. Recovery of Indicators of Mitochondrial Biogenesis, Oxidative Stress, and Aging With (-)-Epicatechin in Senile Mice. J Gerontol A Biol Sci Med Sci N/A:N/A (2014). Mouse . Read more (PubMed: 25143004) »

See all 12 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - 0.1% Triton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Paraformaldehyde
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Submitted Dec 17 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"