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Mouse monoclonal [2E4AD5] to Mitofilin - Mitochondrial Marker
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Mouse, Rat, Cow, Human
Human heart mitochondria.
Human fibroblasts; Human cerebellum tissue; Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; HL60 cells.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.02% Sodium azide
Concentration information loading...
>95% by SDS-PAGE
ab110329 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Light chain type
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Predicted molecular weight: 84 kDa.
Use a concentration of 1 - 5 µg/ml.
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Use a concentration of 1 µg/ml.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use a concentration of 4 µg/ml.
Use at an assay dependent concentration.
Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker images
Western blot - Mitofilin antibody [2E4AD5 ] (ab110329)
All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329) at 2 µg/ml Lane 1 : Isolated mitochondria from Human heart at 5 µg Lane 2 : Isolated mitochondria from Bovine heart at 1 µg Lane 3 : Isolated mitochondria from Rat heart at 10 µg Lane 4 : Isolated mitochondria from Mouse heart at 10 µg Lane 5 : HepG2 cell lysate at 20 µg Predicted band size : 84 kDa Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
Immunocytochemistry/ Immunofluorescence - Mitofilin antibody [2E4AD5 ] (ab110329)
Immunocytochemistry analysis using ab110329 at 5µg/ml staining Mitofilin in Cultured Human fibroblasts. (Fixed and permeabilized) followed by a fluorescent goat-anti-mouse IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mitofilin antibody [2E4AD5 ] (ab110329)
Immunohistological analysis using ab110329 at 1/100 dilution staining Mitofilin in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
Flow Cytometry - Mitofilin antibody [2E4AD5 ] (ab110329)
Flow cytometric analysis using ab110329 at 1µg/ml staining Mitofilin in HL60 cells (blue). Isotype control antibody (red).
References for Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
This product has been referenced in:
et al. Drosophila MIC60/Mitofilin Conducts Dual Roles in Mitochondrial Motility and Crista Structure. Mol Biol Cell N/A:N/A (2017).
Read more (PubMed: 28904209) »
et al. PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling. Elife 6:N/A (2017).
Read more (PubMed: 28463107) »
See all 21 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"