Overview

  • Product name
  • Description
    Rabbit polyclonal to Mitofilin
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Dog, Chimpanzee, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human Mitofilin.

  • Positive control
    • This antibody gave a positive signal in both HepG2 and U87MG whole cell lysates, as well as in the following tissue lysates: Human Liver; Human Heart; Human Brain; Human testis. This antibody gave a positive result in IF in the following methanol fixed cell lines: MCF-7

Properties

Applications

Our Abpromise guarantee covers the use of ab109424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 84 kDa).

Target

  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing protein 52 antibody
    • Cell proliferation-inducing gene 4/52 protein antibody
    • Heart muscle protein antibody
    • HMP antibody
    • IMMT antibody
    • IMMT_HUMAN antibody
    • Inner membrane protein mitochondrial antibody
    • Inner mitochrondial membrane antibody
    • MICOS complex subunit MIC60 antibody
    • MINOS2 antibody
    • Mitochondrial inner membrane organizing system 2 antibody
    • Mitochondrial inner membrane protein antibody
    • Mitofilin antibody
    • Motor protein antibody
    • p87 antibody
    • p87/89 antibody
    • p89 antibody
    • pig4 antibody
    • PIG52 antibody
    • Proliferation-inducing gene 4 antibody
    see all

Images

  • All lanes : Anti-Mitofilin antibody (ab109424) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human heart tissue lysate - total protein (ab29431)
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Human testis tissue lysate - total protein (ab30257)
    Lane 6 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 89 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 77 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab109424 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109424 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab109424 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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