Overview

  • Product nameAnti-Mitofilin antibody
    See all Mitofilin primary antibodies
  • Description
    Rabbit polyclonal to Mitofilin
  • SpecificityThis antibody is specific for Mitofilin
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cat, Dog, Human
  • Immunogen

    Recombinant full length Mitofilin protein (Human)

  • Positive control
    • HeLa whole cell lysate; Dog testis

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab48139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 90-91 kDa (predicted molecular weight: 84 kDa).
ICC/IF 1/500.

Target

Anti-Mitofilin antibody images

  • Immunofluorescence of HeLa cells with ab48139 at 1/500 dilution labeling mitochondria.
  • Anti-Mitofilin antibody (ab48139) at 1/2000 dilution + Dog testis total protein extract

    Predicted band size : 84 kDa
    Observed band size : 87,89 kDa (why is the actual band size different from the predicted?)

References for Anti-Mitofilin antibody (ab48139)

This product has been referenced in:
  • Ilgen P  et al. STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue. PLoS One 9:e101563 (2014). ICC/IF ; Human . Read more (PubMed: 25025184) »
  • Jans DC  et al. STED super-resolution microscopy reveals an array of MINOS clusters along human mitochondria. Proc Natl Acad Sci U S A 110:8936-41 (2013). Read more (PubMed: 23676277) »

See all 8 Publications for this product

Product Wall

Thank you for contacting us.

The sequence you provided and our mitofilin antibody have 71% homology, which is a little low to predict reactivity. Generally at 85% homology with the immunogen or higher with a particular species we predict that ...

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Thank you very much for your patience while I was in discussion with lab.

My colleague has confirmed that, they tested ab57523 with PC-12, Raw 264.7 and NIH/3T3 cells in western blot and they haven't observed any band at 88kDa. The band they o...

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Thank you for contacting us.

Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen secti...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK 293T)
Loading amount 20 µg
Specification HEK 293T
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Dec 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 5 µg
Specification brain
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Dec 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse liver)
Loading amount 25 µg
Specification Mouse liver
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 04 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"