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Synthetic peptide corresponding to a region within C terminal amino acids 683-732 (QVDITQKQLE EEIARLPKEI DQLEKIQNNS KLLRNKAVQL ENELENFTKQ) of Human Mitofusin 1 (NP_284941).
Our Abpromise guarantee covers the use of ab104274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use at an assay dependent concentration.|
ab104274 staining Mitofusin 1 in the H9C2 cell line from Rat heart by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 5% Serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200 in PBS + 5% Goat serum) for 2 hours at 21°C. AnAlexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
This image is courtesy of an anonymous AbreviewBlocking Step: 5% Milk for 1 hour at room temperature