Anti-Mitofusin 1 antibody (ab94508)

Overview

  • Product nameAnti-Mitofusin 1 antibody
    See all Mitofusin 1 primary antibodies
  • Description
    Rabbit polyclonal to Mitofusin 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human Mitofusin 1.

    (Peptide available as ab108243.)

  • Positive control
    • This antibody gave a positive in NIH3T3 and MEF1 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab94508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).

Target

  • FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
  • Tissue specificityUbiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
  • Sequence similaritiesBelongs to the mitofusin family.
  • Post-translational
    modifications
    Ubiquitinated by MARCH5.
  • Cellular localizationCytoplasm and Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fzo homolog antibody
    • Hfzo1 antibody
    • Hfzo2 antibody
    • MFN 1 antibody
    • Mfn1 antibody
    • MFN1_HUMAN antibody
    • Mitochondrial transmembrane GTPase Fzo 1 antibody
    • Mitochondrial transmembrane GTPase FZO 2 antibody
    • Mitochondrial transmembrane GTPase FZO1B antibody
    • Mitofusin 1 antibody
    • Mitofusin-1 antibody
    • Mitofusin1 antibody
    • MS996 antibody
    • Putative transmembrane GTPase antibody
    • Transmembrane GTPase MFN1 antibody
    see all

Anti-Mitofusin 1 antibody images

  • All lanes : Anti-Mitofusin 1 antibody (ab94508) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,55 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-Mitofusin 1 antibody (ab94508)

ab94508 has not yet been referenced specifically in any publications.

Product Wall

Our data for this target says that the human Mitofusin 1 protein has 741 amino acids. Please see the SwissProt site for more information: http://www.uniprot.org/uniprot/Q8IWA4. Because this antibody is a polyclonal antibody, it will recognize multiple ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"