Overview

  • Product name
  • Description
    Rabbit polyclonal to Mitofusin 2
  • Tested applications
    Suitable for: WB, IP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Duck, Penguin
    Predicted to work with: Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide:

    KINGIFEQLGAYIQESAT

    with C-terminal added cysteine, conjugated to KLH, corresponding to N terminal amino acids 38-55 of Human Mitofusin 2

  • Positive control
    • Rat brain mitochondrial lysate; mouse brain mitochondrial lysate; differentiated mouse C2 cells; HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IP Use at an assay dependent concentration.

5-10µg

ICC/IF Use a concentration of 20 - 30 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
  • Tissue specificity
    Ubiquitous; expressed at low level. Highly expressed in heart and kidney.
  • Involvement in disease
    Charcot-Marie-Tooth disease 2A2
    Neuropathy, hereditary motor and sensory, 6A
  • Sequence similarities
    Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
  • Post-translational
    modifications
    Phosphorylated by PINK1.
    Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
  • Cellular localization
    Mitochondrion outer membrane. Colocalizes with BAX during apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMT2A antibody
    • CMT2A2 antibody
    • CPRP 1 antibody
    • CPRP1 antibody
    • EC 3.6.5.- antibody
    • Fzo antibody
    • HSG antibody
    • hyperplasia suppressor gene antibody
    • Hypertension related protein 1 antibody
    • KIAA0214 antibody
    • MARF antibody
    • MFN 2 antibody
    • Mfn2 antibody
    • MFN2_HUMAN antibody
    • Mitochondrial assembly regulatory factor antibody
    • Mitofusin-2 antibody
    • Mitofusin2 antibody
    • Transmembrane GTPase MFN2 antibody
    see all

Images

  • Lane 1 : Anti-Mitofusin 2 antibody (ab50838) at 0.5 µg/ml
    Lanes 2 - 3 : Anti-Mitofusin 2 antibody (ab50838) at 1 µg/ml
    Lane 4 : Anti-Mitofusin 2 antibody (ab50838) at 2 µg/ml

    Lane 1 : rat brain mitochondria extract
    Lane 2 : rat brain mitochondria extract
    Lane 3 : rat brain mitochondria extract with Mitofusin 2 immunizing peptide at 5 µg/ml
    Lane 4 : rat brain mitochondria extract

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate
    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 86 kDa

References

This product has been referenced in:
  • Balboa E  et al. MLN64 induces mitochondrial dysfunction associated with increased mitochondrial cholesterol content. Redox Biol 12:274-284 (2017). WB . Read more (PubMed: 28282615) »
  • Naon D  et al. Critical reappraisal confirms that Mitofusin 2 is an endoplasmic reticulum-mitochondria tether. Proc Natl Acad Sci U S A 113:11249-11254 (2016). WB ; Mouse . Read more (PubMed: 27647893) »

See all 23 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (h9c2)
Specification
h9c2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Chinese Hamster Cell lysate - whole cell (CHO cell)
Specification
CHO cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 10 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (LIVER)
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2014

Thank you for contacting Abcam regarding ab50838.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check th...

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Application
Western blot
Sample
Cairina moschata Cell lysate - other (isolated muscle mitochondria)
Loading amount
50 µg
Specification
isolated muscle mitochondria
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Mar 08 2012

Thank you for contacting us.

The rabbit polyclonal I recommended for IHC with mouse tissue is ab16056, the COX IV antibody. If you already have a COX IV antibody, I recommend trying that first. Antibody ab16056 is not guaranteed for IHC on f...

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Merci de nous avoir contactés. Comme convenu, je vous transfère le numéro de commande de remplacement gratuit du ab50838  : ******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. En échange, nous serions ...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human keratinocytes)
Specification
human keratinocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,25 triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 28 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (human immortalized keratinocytes and cervical tumo)
Loading amount
50 µg
Specification
human immortalized keratinocytes and cervical tumo
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (Isolated brain mitochondria)
Loading amount
20 µg
Specification
Isolated brain mitochondria
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Jia Yao

Verified customer

Submitted Sep 17 2009

1-10 of 11 Abreviews or Q&A

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