Anti-Mitofusin 2 antibody (ab50838)
Key features and details
- Rabbit polyclonal to Mitofusin 2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Mitofusin 2 antibody
See all Mitofusin 2 primary antibodies -
Description
Rabbit polyclonal to Mitofusin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Cynomolgus monkey, Orangutan -
Immunogen
Synthetic peptide:
KINGIFEQLGAYIQESAT
with C-terminal added cysteine, conjugated to KLH, corresponding to N terminal amino acids 38-55 of Human Mitofusin 2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (5) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa). |
Target
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Function
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. -
Tissue specificity
Ubiquitous; expressed at low level. Highly expressed in heart and kidney. -
Involvement in disease
Charcot-Marie-Tooth disease 2A2
Neuropathy, hereditary motor and sensory, 6A -
Sequence similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain. -
Post-translational
modificationsPhosphorylated by PINK1.
Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion. -
Cellular localization
Mitochondrion outer membrane. Colocalizes with BAX during apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 64476 Rat
- SwissProt: Q8R500 Rat
- Unigene: 8570 Rat
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Alternative names
- CMT2A antibody
- CMT2A2 antibody
- CPRP 1 antibody
see all
Images
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Lane 1 : Anti-Mitofusin 2 antibody (ab50838) at 0.5 µg/ml
Lanes 2-3 : Anti-Mitofusin 2 antibody (ab50838) at 1 µg/ml
Lane 4 : Anti-Mitofusin 2 antibody (ab50838) at 2 µg/ml
Lanes 1-2 & 4 : rat brain mitochondria extract
Lane 3 : rat brain mitochondria extract with Mitofusin 2 immunizing peptide at 5 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate
Developed using the ECL technique.
Predicted band size: 86 kDa
Observed band size: 86 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (49)
ab50838 has been referenced in 49 publications.
- Sugiura K et al. MuRF1 deficiency prevents age-related fat weight gain, possibly through accumulation of PDK4 in skeletal muscle mitochondria in older mice. J Orthop Res 40:1026-1038 (2022). PubMed: 34185335
- Jiang C et al. CircTHBS1 facilitates the progression of interstitial cystitis depending on the regulation of miR-139-5p/MFN2 axis. Drug Dev Res 83:351-361 (2022). PubMed: 34368980
- Rossi A et al. Calcium Signaling and Mitochondrial Function in Presenilin 2 Knock-Out Mice: Looking for Any Loss-of-Function Phenotype Related to Alzheimer's Disease. Cells 10:N/A (2021). PubMed: 33494218
- Troiano A et al. ZSCAN4+ mouse embryonic stem cells have an oxidative and flexible metabolic profile. EMBO Rep 21:e48942 (2020). PubMed: 32424995
- Meza-Torres C et al. Resveratrol Regulates the Expression of Genes Involved in CoQ Synthesis in Liver in Mice Fed with High Fat Diet. Antioxidants (Basel) 9:N/A (2020). PubMed: 32429295