The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 84 kDa).
Use at 5-10 µg/mg of lysate. (using HeLa cell lysate)
FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
Tissue specificityUbiquitous; expressed at low level. Highly expressed in heart and kidney.
Involvement in diseaseCharcot-Marie-Tooth disease 2A2 Neuropathy, hereditary motor and sensory, 6A
Sequence similaritiesBelongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily. Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
Post-translational modificationsPhosphorylated by PINK1. Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
Cellular localizationMitochondrion outer membrane. Colocalizes with BAX during apoptosis.
Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody (ab50843)Image courtesy of an anonymous Abreview.
ab50843 staining Mitofusin 2 in murine cardiac myocyte cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permeabilized using 1% Triton X-100, blocked with 10% serum for 1 hour at room temperature, then incubated with ab50843 at a 1/200 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.