Overview

  • Product name
  • Description
    Rabbit polyclonal to Mitofusin 2
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Mitofusin 2 aa 557-576 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    C-LGPKNSRRALMGYNDQVQRP

  • Positive control
    • Mouse brain mitochondrial extract; rat brain mitochondrial extract; HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 84 kDa).
IP Use at 5-10 µg/mg of lysate. (using HeLa cell lysate)

Target

  • Function
    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
  • Tissue specificity
    Ubiquitous; expressed at low level. Highly expressed in heart and kidney.
  • Involvement in disease
    Charcot-Marie-Tooth disease 2A2
    Neuropathy, hereditary motor and sensory, 6A
  • Sequence similarities
    Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
  • Post-translational
    modifications
    Phosphorylated by PINK1.
    Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
  • Cellular localization
    Mitochondrion outer membrane. Colocalizes with BAX during apoptosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMT2A antibody
    • CMT2A2 antibody
    • CPRP 1 antibody
    • CPRP1 antibody
    • EC 3.6.5.- antibody
    • Fzo antibody
    • HSG antibody
    • hyperplasia suppressor gene antibody
    • Hypertension related protein 1 antibody
    • KIAA0214 antibody
    • MARF antibody
    • MFN 2 antibody
    • Mfn2 antibody
    • MFN2_HUMAN antibody
    • Mitochondrial assembly regulatory factor antibody
    • Mitofusin-2 antibody
    • Mitofusin2 antibody
    • Transmembrane GTPase MFN2 antibody
    see all

Images

  • All lanes : Anti-Mitofusin 2 antibody (ab50843) at 0.25 µg/ml

    Lane 1 : Rat brain mitochondria
    Lane 2 : Mouse brain mitochondria

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate.

    Predicted band size : 84 kDa
    Observed band size : 86 kDa (why is the actual band size different from the predicted?)
  • ab50843 staining Mitofusin 2 in murine cardiac myocyte cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using 1% Triton X-100, blocked with 10% serum for 1 hour at room temperature, then incubated with ab50843 at a 1/200 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:
  • Ruiz LM  et al. Quercetin Affects Erythropoiesis and Heart Mitochondrial Function in Mice. Oxid Med Cell Longev 2015:836301 (2015). WB ; Mouse . Read more (PubMed: 26106459) »
  • Fahrenbach JP  et al. Abcc9 is required for the transition to oxidative metabolism in the newborn heart. FASEB J 28:2804-15 (2014). Read more (PubMed: 24648545) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (mouse heart)
Total protein in input
200 µg
Specification
mouse heart
Immuno-precipitation step
Protein G
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Abcam user community

Verified customer

Submitted Feb 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
20 µg
Specification
mouse heart
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Oct 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse cardiac myocyte)
Specification
mouse cardiac myocyte
Fixative
Formaldehyde
Permeabilization
Yes - 1% triton x100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
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Verified customer

Submitted Sep 29 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Loading amount
20 µg
Specification
Mouse Embryonic Fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Dec 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Loading amount
20 µg
Specification
HEK 293T
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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