Anti-Mitotic Cells antibody [4A63] (ab14577)

Overview

  • Product nameAnti-Mitotic Cells antibody [4A63]
    See all Mitotic Cells primary antibodies
  • Description
    Mouse monoclonal [4A63] to Mitotic Cells
  • Tested applicationsSuitable for: ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Tissue/ cell preparation (mitotic cells).

Properties

Applications

Our Abpromise guarantee covers the use of ab14577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceThe life cycle of a eukaryotic cell consists of various phases, two of which can easily be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques. In between the M and S phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent’ phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S and from G2 to M-phase.

References for Anti-Mitotic Cells antibody [4A63] (ab14577)

ab14577 has not yet been referenced specifically in any publications.

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