Anti-Mitotic Cells antibody [8B3G] (ab8956)

Overview

  • Product nameAnti-Mitotic Cells antibody [8B3G]
    See all Mitotic Cells primary antibodies
  • Description
    Mouse monoclonal [8B3G] to Mitotic Cells
  • SpecificityStrongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) this antibody clearly distinguishes these M-phase cells from cell at other stages of the cell cycle. Dynamic information can be obtained by combining BrdU incorporation with antibody staining, which can distinguish and quantitate the four major fractions of the cell cycle. This antibody can be used for flow cytometric analyses and immunocytochemistry, it is not suitable for immunoblotting.
  • Tested applicationsSuitable for: Flow Cyt, ICC, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human, Zebrafish
  • Immunogen

    Total cell lysate of the human bladder carcinoma cell line T24.

Properties

Applications

Our Abpromise guarantee covers the use of ab8956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
ICC
IHC-Fr
  • Application notesFlow Cyt: 1/50 - 1/100.
    ICC: Use at an assay dependent concentration.
    IHC-Fr: Use at an assay dependent concentration. Recommended range is 1:50 - 1:100 for immunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

    Is unsuitable for WB.

    This antibody can be used in flow cytometric analyses of cell suspensions to detect the mitotic index.

    Using immunocytochemistry, a combination of this antibody and BrdU (ab8955) can distinguish and quantitate the four major fractions of the cell cycle.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe life cycle of a eukaryotic cell consists of various phases, two of which can easily be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques. In between the M and S phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent’ phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S and from G2 to M-phase.

    Anti-Mitotic Cells antibody [8B3G] images

    • Dual parameter flow cytometric analysis of human colon cancer HT29 cells stained with monoclonal antibody 8B3G and propidium iodide (PI). The mitotic cell fraction is encircled.

       

    • ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections).Samples ware frozen sections fixed in Acetone:Methanol 1:1. ab8956 used at 1:50 dilution and incubated for 45 minutes at room temperature.
    • ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections).Samples ware frozen sections fixed in Acetone:Methanol 1:1. ab8956 used at 1:50 dilution and incubated for 45 minutes at room temperature.

    References for Anti-Mitotic Cells antibody [8B3G] (ab8956)

    This product has been referenced in:
    • Delobel P  et al. Cell-cycle markers in a transgenic mouse model of human tauopathy: increased levels of cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1. Am J Pathol 168:878-87 (2006). Mouse . Read more (PubMed: 16507903) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Apteronotus leptorhynchus Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization Yes - 0.3% TritonX-100
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 05 2013

    Thank you very much for your reply and providing this additional information. We appreciate the time that was spent providing this Abreview.
    The Abreview has now been published and you have received the reward points.
    Please do not hesitate to ...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount 50 µg
    Specification Brain
    Gel Running Conditions Reduced Denaturing (4-15%)
    Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 06 2012

    The mice that were used to produce ab8956 were indeed immunized with a total cell lysate of proliferating T24. Since the antibody behaves rather poorly in immunoblotting or the amount of its antigen in the cell is low, we have not been able to clearly ...

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    Thank you for your patience in awaiting a response. Unfortunately this antibody acts poorly in immunoblotting studies. We therefore have not been able to identify the antigen that the antibody recognizes. Immunofluorescence studies and flow cytometric ...

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    Thank you for your enquiry. Although suitable for FACS, due to the amount supplied I have removed this as a tested application from the on line data sheet. If you have any further questions then please do not hesitate to get back in touch with us. I ap...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"