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Product name
MitoTox™ Complex I OXPHOS Activity Microplate Assay
See all Complex I products (7) ...
Tests
1 x 96 well plate
Assay type
Quantitative
Assay time
5h 00m
Cross reactivity
Reacts with
Cow
Product overview
ab109903 MitoTox™ Complex I OXPHOS Activity Microplate Assay is designed for screening the direct inhibitory effect of compounds on Complex I activity and does this using enzyme immunocaptured from bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex I. The phospholipids provided in the kit are essential for rotenone-sensitive Complex I activity, rotenone being a well-known inhibitor of Complex I.
Notes
Store Microplate, Wash buffer, Mito Buffer, and detergent at 4°C. Reagent reservoirs may be stored at RT. Store Phospholipids at -20°C. Store Bovine heart mitochondria, Ubiquinone and Activity Buffer at -80°C.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Please see Notes section
| Components | 96 tests |
|---|---|
| 12-channel reagent reservoirs | 2 units |
| 1X Mito Buffer | 1 x 5ml |
| 20X Wash Buffer | 1 x 5ml |
| Bovine heart mitochondria | 1 x 360µl |
| Complex I Activity Buffer | 1 x 24ml |
| Detergent | 1 x 100µl |
| Phospholipids | 1 x 6ml |
| Pre-coated 96-well microplate | 1 unit |
| Ubiquinone 1 | 1 x 60µl |
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Functional Studies - MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab109903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS
MitoTox™ Complex I OXPHOS Activity Microplate Assay images:
Functional Studies - MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903)
Dose Response Assay using Rotenone
Protocols
References for MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903)
ab109903 has not yet been referenced specifically in any publications.
Publishing research using ab109903? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"