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MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904)

MSCatalog No. MTOX2

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    Overview

    Product name

    MitoTox™ Complex II OXPHOS Activity Microplate Assay
    See all Complex II products (5) ...

    Tests

    1 x 96 well plate

    Assay type

    Direct

    Assay time

    4h 00m

    Cross reactivity

    Reacts with

    Cow

    Product overview

    MitoTox™ Complex II OXPHOS Activity Microplate Assay is designed for testing the direct inhibitory effect of compounds on Complex II activity using enzyme immunocaptured from Bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex II.



    Notes

    Please store Succinate, Ubiquinone 2, BHM, DCPIP at -80°C and all other components at 4°C.

    Tested applications

    Functional Studiesmore details

    Properties

    Storage instructions

    Please see Notes section

    Components 96 tests
    10X Detergent1 x 1ml
    12-channel reagent reservoirs2 units
    20 x Buffer1 x 5ml
    Bovine heart mitochondria 2 x 360µl
    Complex II Activity Buffer1 x 24ml
    DCPIP1 x 250µl
    Pre-coated 96-well microplate1 x 96 tests
    Succinate1 x 500µl
    Ubiquinone 21 x 60µl

    Research areas

    Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
    Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
    Signal Transduction >> Metabolism >> Mitochondrial

    • Functional Studies - MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904)Functional Studies - MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904) image (enlarge)

    Relevance

    Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma.

    Alternative names

    • MS1036
    • MS241
    • MTOX2
    • Succinate coenzyme Q reductase

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab109904 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 1 Image

      Functional Studies

       FuncS

      FuncS

    MitoTox™ Complex II OXPHOS Activity Microplate Assay images:

      Functional Studies - MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904)

    Functional Studies - MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904)

    Protocols

  • Protocol Booklet

    • References for MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904)

    ab109904 has not yet been referenced specifically in any publications.

    Publishing research using ab109904? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"