Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
MitoTox™ Complex IV OXPHOS Activity Microplate Assay
See all Complex IV products (10) ...
Tests
1 x 96 test
Assay type
Direct
Assay time
5h 00m
Cross reactivity
Reacts with
Cow
Product overview
ab109906 MitoTox™ OXPHOS Complex IV Activity Kit (MTOX4) is designed for screening the direct inhibitory effect of compounds on Complex IV activity and does this using enzyme immunocaptured from Bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex IV.
Notes
Store at -80°C: Bovine heart mitochondria
Store at -20°C: Reagent C (store at -80°C for long term storage)
Store at 4°C: Pre-coated 96-well plate, Blocking Buffer, Mito Buffer, Detergent
Store at room temperature or 4°C: Blocking powder and reagent reservoirs
Store at -20°C: Reagent C (store at -80°C for long term storage)
Store at 4°C: Pre-coated 96-well plate, Blocking Buffer, Mito Buffer, Detergent
Store at room temperature or 4°C: Blocking powder and reagent reservoirs
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Please see Notes section
| Components | 96 tests |
|---|---|
| 12-channel reagent reservoirs | 2 units |
| 1X Blocking Buffer (Solution 1) | 1 x 70ml |
| 1X Complex IV Mito Buffer (Solution 2) | 1 x 110ml |
| Bovine Heart Mitochondria | 1 x 90µl |
| Detergent | 1 x 20µl |
| Fat free powdered milk (Blocking powder) | 1 x 1.8g |
| Pre-coated 96-well microplate | 1 unit |
| Reagent C (reduced cytochrome c) | 2 x 1ml |
Research areas
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Signal Transduction >> Metabolism >> Mitochondrial
-
Functional Studies - MitoTox™ Complex IV OXPHOS Activity Microplate Assay (ab109906)
(enlarge)
Alternative names
- Cytochrome c oxidase
- MS1038
- MS441
- MTOX4
Applications
Show applications key
Our Abpromise guarantee covers the use of ab109906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS
MitoTox™ Complex IV OXPHOS Activity Microplate Assay images:
Protocols
References for MitoTox™ Complex IV OXPHOS Activity Microplate Assay (ab109906)
ab109906 has not yet been referenced specifically in any publications.
Publishing research using ab109906? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"