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MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903) is designed for screening the direct inhibitory effect of compounds on Complex I activity and does this using enzyme immunocaptured from bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex I. The phospholipids provided in the kit are essential for rotenone-sensitive Complex I activity, rotenone being a well-known inhibitor of Complex I.
Store Microplate, Wash buffer, Mito Buffer, and detergent at 4°C. Reagent reservoirs may be stored at RT. Store Phospholipids at -20°C. Store Bovine heart mitochondria, Ubiquinone and Activity Buffer at -80°C.
|12-channel reagent reservoirs||2 units|
|1X Mito Buffer||1 x 5ml|
|20X Wash Buffer||1 x 5ml|
|Bovine heart mitochondria||1 x 360µl|
|Complex I Activity Buffer||1 x 24ml|
|Detergent||1 x 100µl|
|Phospholipid||1 x 6ml|
|Pre-coated 96-well microplate||1 unit|
|Ubiquinone 1||1 x 60µl|
Our Abpromise guarantee covers the use of ab109903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|