MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904)


  • Product nameMitoTox™ Complex II OXPHOS Activity Microplate Assay
    See all Complex II kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Assay typeDirect
  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Product overview

    MitoTox™ Complex II OXPHOS Activity Microplate Assay is designed for testing the direct inhibitory effect of compounds on Complex II activity using enzyme immunocaptured from Bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex II.

  • Notes

    Please store Succinate, Ubiquinone 2, BHM, DCPIP at -80°C and all other components at 4°C.

  • Tested applicationsSuitable for: Functional Studiesmore details
  • PlatformMicroplate



Our Abpromise guarantee covers the use of ab109904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

MitoTox™ Complex II OXPHOS Activity Microplate Assay images


References for MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904)

This product has been referenced in:
  • Landry GM  et al. Diglycolic acid inhibits succinate dehydrogenase activity in human proximal tubule cells leading to mitochondrial dysfunction and cell death. Toxicol Lett 221:176-84 (2013). Read more (PubMed: 23827505) »
  • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

See all 2 Publications for this product

Product Wall

The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
ab109903, Complex I; reactivity = Human, mouse, rat, bovine

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ab109904 measures solely complex II, whereas ab109905 measures complexes II+III.
We do not offer an immunocapture activity assay for Complex III only. The reason is that we have not generated an antibody capable of immunocapturing the native enz...

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Thank you for contacting us.

The 10x detergent in the kit is 10% Lauryl maltoside, which can also be purchased separately as ab109857.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any...

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Thank you for your call and for your patience while I've been in touch with the lab about ab109904.

My contact at the lab gave the following reply: "Yes, a pH of 2 will destroy the assay. You will need to bring the pH to neutral ( 7 &nda...

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