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MitoTox™ Complex II OXPHOS Activity Microplate Assay is designed for testing the direct inhibitory effect of compounds on Complex II activity using enzyme immunocaptured from Bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex II.
Please store Succinate, Ubiquinone 2, BHM, DCPIP at -80°C and all other components at 4°C.
|10X Detergent||1 x 1ml|
|12-channel reagent reservoirs||2 units|
|20 x Buffer||1 x 5ml|
|Bovine heart mitochondria||2 x 360µl|
|Complex II Activity Buffer||1 x 24ml|
|DCPIP||1 x 250µl|
|Pre-coated 96-well microplate||1 x 96 tests|
|Succinate||1 x 500µl|
|Ubiquinone 2||1 x 60µl|
Our Abpromise guarantee covers the use of ab109904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|