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ab109906 MitoTox™ OXPHOS Complex IV Activity Kit (MTOX4) is designed for screening the direct inhibitory effect of compounds on Complex IV activity and does this using enzyme immunocaptured from Bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex IV.
Store at -80°C: Bovine heart mitochondria
Store at -20°C: Reagent C (store at -80°C for long term storage)
Store at 4°C: Pre-coated 96-well plate, Blocking Buffer, Mito Buffer, Detergent
Store at room temperature or 4°C: Blocking powder and reagent reservoirs
|12-channel reagent reservoirs||2 units|
|1X Blocking Buffer (Solution 1)||1 x 70ml|
|1X Complex IV Mito Buffer (Solution 2)||1 x 110ml|
|Bovine Heart Mitochondria||1 x 90µl|
|Detergent||1 x 20µl|
|Fat free powdered milk (Blocking powder)||1 x 1.8g|
|Pre-coated 96-well microplate||1 unit|
|Reagent C (Cytochrome c)||2 x 1ml|
Our Abpromise guarantee covers the use of ab109906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|