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The MitoTox™ Complex V OXPHOS Activity Microplate Assay is designed for testing the direct inhibitory effect of compounds on Complex V activity using enzyme immunocaptured from bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex V. The phospholipids provided in this kit are essential for Complex V activity.
Pre-coated 96-well microplate, Mito buffer, Wash Buffer and detergent should be stored at 4°C.Phopholipids should be stored at -20°C. Bovine heart mitochondria and Activity buffer should be stored at -80°C. Reagent reservoirs should be stored at room temperature.
|12-channel reagent reservoirs||2 units|
|1X Mito Buffer||1 x 5ml|
|20X Wash Buffer||1 x 5ml|
|Bovine heart mitochondria||1 x 360µl|
|Complex V Activity Buffer||1 x 24ml|
|Detergent||1 x 100µl|
|Phospholipid||1 x 6ml|
|Pre-coated 96-well microplate||1 unit|
Our Abpromise guarantee covers the use of ab109907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|