MitoTox™ Complex IV OXPHOS Activity Assay Kit (ab109906)

Overview

  • Product name
    MitoTox™ Complex IV OXPHOS Activity Assay Kit
    See all Complex IV kits
  • Detection method
    Colorimetric
  • Assay type
    Direct
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    MitoTox™ Complex IV OXPHOS Activity Assay Kit (ab109906) is designed for testing the direct inhibitory effect of compounds on Complex IV activity in only 5 hours. Complex IV extracted from the provided bovine heart mitochondria (a rich source of Complex IV) is immunocaptured by specific antibodies on the plate. Complex IV activity can be observed as decrease in absorbance at OD 550 nm. The intra-assay and inter-assay variation of this assay are both <10%.


    Inhibitory effects of compounds on Complex IV activity can be tested in two different ways: 1. Screening format, where up to 23 compounds can be tested at a single concentration in triplicate; 2. Dose response (IC50) format, where two compounds known to affect Complex IV activity can be tested at 11 different data points in triplicate.


    Testing for mitochondrial function has become a key aspect of drug discovery. Mitochondria can be affected by drug treatment, resulting into cardio- and hepatotoxic side effects that can lead to drug withdrawal from the market. Therefore, there is increasing emphasis on testing the impact on mitochondria early on in the drug development process to reduce failure rates during preclinical and clinical phases. 


     


     

  • Notes

    Store Bovine Heart Mitochondria and Reagent C at -80°C. Store all other components at 4°C.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab109906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

MitoTox™ Complex IV OXPHOS Activity Assay Kit images

  • Typical dose response curve for KCN. Assay was performed following the Dose Response Assay Procedure using KCN, a well known Complex IV inhibitor. KCN was prepared in 0.1 M NaOH to generate a 100 mM stock. Starting with a 100 µM final concentration in well, 1:10 serial dilutions of KCN were generated.

Protocols

References for MitoTox™ Complex IV OXPHOS Activity Assay Kit (ab109906)

This product has been referenced in:
  • Chin RM  et al. The metabolite a-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR. Nature 509:397-401 (2014). Read more (PubMed: 24828042) »
  • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

See all 2 Publications for this product

Product Wall

The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
ab109903, Complex I; reactivity = Human, mouse, rat, bovine
http://www.abcam.com/mitotox%E2%84%A2-complex-i...

Read More

This is because the plate requires fresh blocking to prevent non specific binding of the enzyme to the plate. We believe that this occurs because CIV is a highly hydrophobic enzyme.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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