MitoSciences (ab109906)

MitoTox™ Complex IV OXPHOS Activity Microplate Assay (ab109906)

Overview

  • Product nameMitoTox™ Complex IV OXPHOS Activity Microplate AssaySee all Complex IV kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 test
  • Assay typeDirect
  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Cow
  • Product overview

    ab109906 MitoTox™ OXPHOS Complex IV Activity Kit (MTOX4) is designed for screening the direct inhibitory effect of compounds on Complex IV activity and does this using enzyme immunocaptured from Bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex IV.

  • Notes

    Store at -80°C: Bovine heart mitochondria

    Store at -20°C: Reagent C (store at -80°C for long term storage)

    Store at 4°C: Pre-coated 96-well plate, Blocking Buffer, Mito Buffer, Detergent

    Store at room temperature or 4°C: Blocking powder and reagent reservoirs

  • Tested applicationsFunctional Studies more details
  • PlatformMicroplate

Properties

  • Alternative names
    • COX
    • Cytochrome c oxidase
    • Cytochrome oxidase

Applications

Our Abpromise guarantee covers the use of ab109906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

MitoTox™ Complex IV OXPHOS Activity Microplate Assay images

Protocols

References for MitoTox™ Complex IV OXPHOS Activity Microplate Assay (ab109906)

This product has been referenced in:
  • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

See 1 Publication for this product

Product Wall

This is because the plate requires fresh blocking to prevent non specific binding of the enzyme to the plate. We believe that this occurs because CIV is a highly hydrophobic enzyme.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"