The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression.
Restricted to progenitors and secondary lymph tissues. In normal hematopoiesis, it is restricted to immature B-and T-lymphoid cells. Present in differentiating embryonic stem cells (at protein level).
Belongs to the paired homeobox family. Contains 1 homeobox DNA-binding domain.