Overview

  • Product nameAnti-Mixl1 antibody
    See all Mixl1 primary antibodies
  • Description
    Rabbit polyclonal to Mixl1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity

    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Mouse Mixl1.

    (Peptide available as ab30617.)

  • Positive control
    • This antibody gave a positive signal in Murine ES WCL expressing mesoderm Marker lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • FunctionTranscription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression.
  • Tissue specificityRestricted to progenitors and secondary lymph tissues. In normal hematopoiesis, it is restricted to immature B-and T-lymphoid cells. Present in differentiating embryonic stem cells (at protein level).
  • Sequence similaritiesBelongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated at multiple sites.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hMix antibody
    • Homeobox protein MIXL1 antibody
    • Homeodomain protein 1 antibody
    • Homeodomain protein MIX antibody
    • MILD1 antibody
    • MIX antibody
    • Mix.1 antibody
    • Mix.1 homeobox-like protein antibody
    • Mix1 homeobox-like 1 antibody
    • MIX1 homeobox-like protein 1 antibody
    • MIXL antibody
    • Mixl1 antibody
    • MIXL1_HUMAN antibody
    see all

Anti-Mixl1 antibody images

  • Anti-Mixl1 antibody (ab28411) at 1 µg/ml + Whole Cell Lysate from Murine ES Cells Differentiated to Express Mesoderm Markers at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Mixl1 antibody (ab28411)

ab28411 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Day 4 differentiated Mouse Embryonic Stem Cells)
Specification Day 4 differentiated Mouse Embryonic Stem Cells
Fixative Formaldehyde
Permeabilization Yes - PBS-0.3% Tween
Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Dr. Vladimir Mazurov

Verified customer

Submitted Aug 25 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E6.5 embryo)
Specification E6.5 embryo
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization Yes - Tween
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Ms. Mary Trask

Verified customer

Submitted Jan 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"