Overview

  • Product nameAnti-Mkl1 antibody
    See all Mkl1 primary antibodies
  • Description
    Rabbit polyclonal to Mkl1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 700 and 750 of Mkl1 using the numbering given in entry NP_065882.1.

  • Positive control
    • Human Breast Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab113264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTranscriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.
  • Tissue specificityUbiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.
  • Involvement in diseaseNote=A chromosomal aberration involving MKL1 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with RBM15. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
  • Sequence similaritiesContains 2 RPEL repeats.
    Contains 1 SAP domain.
  • DomainThe N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.
    The RPEL repeats mediate binding to globular actin.
  • Cellular localizationCytoplasm. Nucleus. Binding to globular actin is required to maintain cytoplasmic localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI852829 antibody
    • AMKL antibody
    • AW743281 antibody
    • AW821984 antibody
    • Basic SAP coiled-coil transcription activator antibody
    • Bsac antibody
    • KIAA1438 antibody
    • Mal antibody
    • Megakaryoblastic leukemia 1 protein antibody
    • Megakaryocytic acute leukemia protein antibody
    • MKL (megakaryoblastic leukemia)/myocardin like 1 antibody
    • MKL/myocardin like protein 1 antibody
    • MKL/myocardin-like protein 1 antibody
    • Mkl1 antibody
    • MKL1_HUMAN antibody
    • MRTF A antibody
    • Mrtf A antibody
    • MRTF-A antibody
    • MRTFA antibody
    • MYOCARDIN RELATED PROTEIN antibody
    • Myocardin related transcription factor A antibody
    • Myocardin-related transcription factor A antibody
    see all

Anti-Mkl1 antibody images

  • Formalin-fixed, paraffin-embedded section of Human breast carcinoma.
    Antibody: ab113264 used at a dilution of 1/250.
    Detection: DAB staining.

References for Anti-Mkl1 antibody (ab113264)

ab113264 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"