• Product nameAnti-Mkl1 antibody
    See all Mkl1 primary antibodies
  • Description
    Rabbit polyclonal to Mkl1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Mkl1.

  • Positive control
    • This antibody gave a positive signal in HepG2 and HEK293 as well as the following tissue lysates: Mouse Lung; Human Placenta; Human Small Intestine. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MEF1



Our Abpromise guarantee covers the use of ab115319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionTranscriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.
  • Tissue specificityUbiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.
  • Involvement in diseaseNote=A chromosomal aberration involving MKL1 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with RBM15. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
  • Sequence similaritiesContains 2 RPEL repeats.
    Contains 1 SAP domain.
  • DomainThe N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.
    The RPEL repeats mediate binding to globular actin.
  • Cellular localizationCytoplasm. Nucleus. Binding to globular actin is required to maintain cytoplasmic localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI852829 antibody
    • AMKL antibody
    • AW743281 antibody
    • AW821984 antibody
    • Basic SAP coiled-coil transcription activator antibody
    • Bsac antibody
    • KIAA1438 antibody
    • Mal antibody
    • Megakaryoblastic leukemia 1 protein antibody
    • Megakaryocytic acute leukemia protein antibody
    • MKL (megakaryoblastic leukemia)/myocardin like 1 antibody
    • MKL/myocardin like protein 1 antibody
    • MKL/myocardin-like protein 1 antibody
    • Mkl1 antibody
    • MKL1_HUMAN antibody
    • MRTF A antibody
    • Mrtf A antibody
    • MRTF-A antibody
    • MRTFA antibody
    • Myocardin related transcription factor A antibody
    • Myocardin-related transcription factor A antibody
    see all

Anti-Mkl1 antibody images

  • ICC/IF image of ab115319 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115319 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Mkl1 antibody (ab115319) at 1 µg/ml

    Lane 1 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 22 kDa,35 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-Mkl1 antibody (ab115319)

ab115319 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab115319.
Please use the links above to contact us or submit feedback about this product.