The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
FunctionE3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
PathwayProtein modification; protein ubiquitination.
Lane 1 : Anti-MKRN1 antibody (ab123804) at 0.3 µg/ml Lane 2 : Anti-MKRN1 antibody (ab123804) at 1 mg/ml Lane 3 : anti-DYKDDDDK Tag anntibody at 1/1000 dilution
Lane 1 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer Lane 2 : HEK293 lysate, mock-transfected Lane 3 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer