Overview

  • Product name
  • Description
    Goat polyclonal to MKRN1
  • Specificity
    ab123804 is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    RYEHSKPLKQEEAT

    by a Cysteine residue linker, corresponding to internal sequence amino acids 105-118 of Human MKRN1 (NP_038474.2; NP_001138597.1).

  • Positive control
    • MKRN1 transfected HEK293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab123804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).

Target

  • Function
    E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
  • Tissue specificity
    Ubiquitous.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 4 C3H1-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Auto-ubiquitinated; which leads to proteasomal degradation.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase makorin-1 antibody
    • FLJ21334 antibody
    • HGNC:7112 antibody
    • Makorin antibody
    • Makorin ring finger protein 1 antibody
    • MKRN 1 antibody
    • mkrn1 antibody
    • MKRN1_HUMAN antibody
    • OTTHUMP00000212117 antibody
    • OTTHUMP00000212118 antibody
    • OTTHUMP00000212121 antibody
    • OTTHUMP00000212124 antibody
    • Ring finger protein 1 antibody
    • RING finger protein 61 antibody
    • RNF61 antibody
    see all

Images

  • All lanes : Anti-MKRN1 antibody (ab123804) at 1 µg/ml

    Lane 1 : fetal mouse brain tissue lysate
    Lane 2 : adult mouse brain tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa (possible isoform).
  • Lane 1 : Anti-MKRN1 antibody (ab123804) at 0.3 µg/ml
    Lane 2 : Anti-MKRN1 antibody (ab123804) at 1 mg/ml
    Lane 3 : anti-DYKDDDDK Tag anntibody at 1/1000 dilution

    Lane 1 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer
    Lane 2 : HEK293 lysate, mock-transfected
    Lane 3 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References

ab123804 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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