Overview

  • Product nameAnti-MKRN1 antibody
    See all MKRN1 primary antibodies
  • Description
    Rabbit polyclonal to MKRN1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 432 and the C-terminus (residue 482) of human MKRN1 (NP_038474.1).

  • Positive control
    • Whole cell lysates from HeLa and 293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionE3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
  • Tissue specificityUbiquitous.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 4 C3H1-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Auto-ubiquitinated; which leads to proteasomal degradation.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase makorin-1 antibody
    • FLJ21334 antibody
    • HGNC:7112 antibody
    • Makorin antibody
    • Makorin ring finger protein 1 antibody
    • MKRN 1 antibody
    • mkrn1 antibody
    • MKRN1_HUMAN antibody
    • OTTHUMP00000212117 antibody
    • OTTHUMP00000212118 antibody
    • OTTHUMP00000212121 antibody
    • OTTHUMP00000212124 antibody
    • Ring finger protein 1 antibody
    • RING finger protein 61 antibody
    • RNF61 antibody
    see all

Anti-MKRN1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MKRN1 with ab72054 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MKRN1 antibody (ab72054) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 100 kDa,16 kDa,28 kDa,45 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MKRN1 antibody (ab72054)

This product has been referenced in:
  • Panneer Selvam S  et al. Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation. Sci Signal 8:ra58 (2015). Read more (PubMed: 26082434) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"