Overview

  • Product name
  • Description
    Rabbit polyclonal to MKRN1
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide: FDNDEEEVVT FELGEMLLML LAAGGDDELT DSEDEWDLFH DELEDFYDLD L, corresponding to C terminal amino acids 432 - 482 of Human MKRN1 (NP_038474.1)

  • Positive control
    • Human tissues - Breast Carcinoma and Lung Carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab93773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended.
    Epitope exposure with tris-EDTA pH 9.0 buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
    • Tissue specificity
      Ubiquitous.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Contains 4 C3H1-type zinc fingers.
      Contains 1 RING-type zinc finger.
    • Post-translational
      modifications
      Auto-ubiquitinated; which leads to proteasomal degradation.
    • Information by UniProt
    • Database links
    • Alternative names
      • E3 ubiquitin-protein ligase makorin-1 antibody
      • FLJ21334 antibody
      • HGNC:7112 antibody
      • Makorin antibody
      • Makorin ring finger protein 1 antibody
      • MKRN 1 antibody
      • mkrn1 antibody
      • MKRN1_HUMAN antibody
      • OTTHUMP00000212117 antibody
      • OTTHUMP00000212118 antibody
      • OTTHUMP00000212121 antibody
      • OTTHUMP00000212124 antibody
      • Ring finger protein 1 antibody
      • RING finger protein 61 antibody
      • RNF61 antibody
      see all

    Images

    • ab93773, at a 1/100 dilution, staining MKRN1 in a Formalin-Fixed Paraffin-Embedded section of Human breast carcinoma, using DAB staining and epitope retrieval with a high pH buffer.
    • ab93773, at a 1/500 dilution, staining MKRN1 in a Formalin-Fixed Paraffin-Embedded section of Human lung carcinoma, using DAB staining and epitope retrieval with a high pH buffer.

    References

    ab93773 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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