Overview

  • Product nameAnti-MKRN2 antibody
    See all MKRN2 primary antibodies
  • Description
    Rabbit polyclonal to MKRN2
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 366 and the C-terminus (residue 416) of human MKRN2 (NP_054879.3).

  • Positive control
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 47 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Anti-MKRN2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MKRN2 with ab72055 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MKRN2 antibody (ab72055) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg


    Predicted band size : 47 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human MKRN2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72055 at 3 µg/mg lysate (Lane 1). Lane 2 represents Control IgG IP. Subsequent Western blot detection was done using ab72055 at 1 µg/ml.

References for Anti-MKRN2 antibody (ab72055)

ab72055 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab72055.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"