Anti-MLF1 Interacting Protein/PBIP1 (phospho T78) antibody (ab34911)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab34911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 48 kDa.
ELISA 1/5000 - 1/25000.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionComponent of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.
  • Tissue specificityExpressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.
  • Post-translational
    modifications
    Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.
  • Cellular localizationCytoplasm. Nucleus. Chromosome > centromere > kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates.
  • Information by UniProt
  • Database links
  • Alternative names
    • CENP U antibody
    • CENP-50 antibody
    • CENP-U antibody
    • CENP50 antibody
    • CENPU antibody
    • CENPU_HUMAN antibody
    • CENPU50 antibody
    • centromere protein of 50 kDa antibody
    • centromere protein U antibody
    • FLJ23468 antibody
    • HHV8 LNAIP1 antibody
    • ICEN24 antibody
    • Interphase centromere complex protein 24 antibody
    • Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1 antibody
    • KLIP-1 antibody
    • KLIP1 antibody
    • KSHV latent nuclear antigen interacting protein 1 antibody
    • KSHV latent nuclear antigen-interacting protein 1 antibody
    • KSHV LNAIP1 antibody
    • LNAIP 1 antibody
    • LNAIP-1 antibody
    • MLF1-interacting protein antibody
    • MLF1IP antibody
    • MLF1IP1 antibody
    • PBIP1 antibody
    • Polo-box-interacting protein 1 antibody
    see all

Anti-MLF1 Interacting Protein/PBIP1 (phospho T78) antibody images

  • ab34911 at 20 µg/ml staining mitotic cells in colon adenocarcinoma at 60X. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

References for Anti-MLF1 Interacting Protein/PBIP1 (phospho T78) antibody (ab34911)

This product has been referenced in:
  • Porter IM  et al. Bod1 regulates protein phosphatase 2A at mitotic kinetochores. Nat Commun 4:2677 (2013). ICC/IF ; Human . Read more (PubMed: 24157919) »
  • Ishiguro K  et al. A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing. EMBO Rep 12:267-75 (2011). ICC/IF ; Mouse . Read more (PubMed: 21274006) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.4% Triton-X
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Jul 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"