Overview

  • Product name
  • Description
    Mouse polyclonal to MLF2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human MLF2 protein (NP_005430.1, 1 a.a. - 248 a.a).

  • Positive control
    • MLF2 transfected 293T cell lysate. Human liver lysate. HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the MLF family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MLF 2 antibody
    • MLF2 antibody
    • MLF2_HUMAN antibody
    • Myelodysplasia myeloid leukemia factor 2 antibody
    • Myelodysplasia-myeloid leukemia factor 2 antibody
    • Myeloid leukemia factor 2 antibody
    • NTN 4 antibody
    • NTN4 antibody
    see all

Images

  • Anti-MLF2 antibody (ab88442) at 1 µg/ml + human liver lysate at 50 µg

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • All lanes : Anti-MLF2 antibody (ab88442) at 1 µg/ml

    Lane 1 : MLF2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of MLF2 expression in HeLa cells using 10 ug/ml ab88442.

References

ab88442 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab88442.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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