Overview

  • Product name
  • Description
    Rabbit polyclonal to MLK2
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human MLK2, conjugated to KLH.

  • Positive control
    • MLK2 transfected COS7 cell lysate, human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab70859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IHC-P
  • Application notes
    ELISA: 1/1000.
    IHC-P: 1/50 - 1/1000.
    WB: 1/100 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa). Detection of endogenous proteins has not yet been validated.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Activates the JUN N-terminal pathway.
    • Tissue specificity
      Expressed in brain and skeletal muscle.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications
      Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • M3K10_HUMAN antibody
      • MAP3K 10 antibody
      • Map3k10 antibody
      • MEKK10 antibody
      • Mitogen activated protein kinase kinase kinase 10 antibody
      • Mitogen-activated protein kinase kinase kinase 10 antibody
      • Mixed lineage kinase 2 antibody
      • MKN28 derived nonreceptor type serine/threonine kinase antibody
      • MKN28 kinase antibody
      • MLK 2 antibody
      • MST antibody
      • Protein kinase MST antibody
      see all

    Images

    • Anti-MLK2 antibody (ab70859) at 1/100 dilution + transfected COS7 cell lysate at 12.5 µg

      Predicted band size : 103 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with 1/50 ab70859. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.

    References

    ab70859 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70859.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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