The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/1000.
IHC-P: 1/50 - 1/1000.
WB: 1/100 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa). Detection of endogenous proteins has not yet been validated.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActivates the JUN N-terminal pathway.
Tissue specificityExpressed in brain and skeletal muscle.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain. Contains 1 SH3 domain.
Post-translational modificationsAutophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.