Overview

  • Product name
    Anti-MLK3 antibody [EP1460Y]
    See all MLK3 primary antibodies
  • Description
    Rabbit monoclonal [EP1460Y] to MLK3
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-Fr,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus of human MLK3.

  • Positive control
    • A431 cell lysate or HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab51068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/15.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-Fr,IHC-P or IP.
  • Target

    • Function
      Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
    • Tissue specificity
      Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications
      Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
    • Cellular localization
      Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2610017K16Rik antibody
      • EC 2.7.11.25 antibody
      • M3K11_HUMAN antibody
      • Map3k11 antibody
      • MEKK11 antibody
      • MGC17114 antibody
      • Mitogen-activated protein kinase kinase kinase 11 antibody
      • Mixed lineage kinase 3 antibody
      • Mixed lineage protein kinase 3 antibody
      • Mlk-3 antibody
      • Mlk3 antibody
      • Protein tyrosine kinase PTK1 antibody
      • PTK1 antibody
      • RHOE antibody
      • SH3 domain containing proline rich kinase antibody
      • SPRK antibody
      • Src-homology 3 domain-containing proline-rich kinase antibody
      see all

    Images



    • Predicted band size : 93 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MLK3 knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab51068 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab51068 was shown to specifically react with MLK3 when MLK3 knockout samples were used. Wild-type and MLK3 knockout samples were subjected to SDS-PAGE. ab51068 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-MLK3 antibody [EP1460Y] (ab51068) at 1/5000 dilution + A431 cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 93 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using ab51068 (1:100).

    References

    This product has been referenced in:
    • Marusiak AA  et al. Mixed lineage kinases activate MEK independently of RAF to mediate resistance to RAF inhibitors. Nat Commun 5:3901 (2014). WB ; Human . Read more (PubMed: 24849047) »
    • Li CH  et al. Zinc oxide nanoparticles-induced intercellular adhesion molecule 1 expression requires Rac1/Cdc42, mixed lineage kinase 3, and c-Jun N-terminal kinase activation in endothelial cells. Toxicol Sci 126:162-72 (2012). Read more (PubMed: 22166487) »

    See all 5 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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