Overview

  • Product name
    Anti-MLK4 antibody - N-terminal
    See all MLK4 primary antibodies
  • Description
    Rabbit polyclonal to MLK4 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human MLK4 conjugated to KLH.

  • Positive control
    • 293 cell lysates transiently transfected with the MLK4 gene. Human colon carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab71510 is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 114 kDa.

Target

  • Function
    Activates the JUN N-terminal pathway.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
  • Information by UniProt
  • Database links
    • Alternative names
      • dJ862P8.3 antibody
      • KIAA1804 antibody
      • M3KL4_HUMAN antibody
      • Mitogen activated protein kinase kinase kinase antibody
      • Mitogen-activated protein kinase kinase kinase MLK4 antibody
      • Mixed lineage kinase 4 antibody
      • MLK 4 antibody
      • Mlk4 antibody
      • RP5 862P8.2 antibody
      see all

    Images

    • Western blot - MLK4 antibody - N-terminal (ab71510)
      Western blot - MLK4 antibody - N-terminal (ab71510)
      All lanes : Anti-MLK4 antibody - N-terminal (ab71510) at 1/50 dilution

      Lane 1 : Nontransfected 293 cell lysate
      Lane 2 : 293 cell lysate transiently transfected with the MLK4 gene

      Lysates/proteins at 2 µg per lane.

      Predicted band size: 114 kDa
      Observed band size: 105 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 130 kDa, 50 kDa, 72 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLK4 antibody - N-terminal (ab71510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLK4 antibody - N-terminal (ab71510)
      ab71510 at 1/10 dilution, staining MLK4 in human colon carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

    References

    ab71510 has not yet been referenced specifically in any publications.

    Publishing research using ab71510? Please let us know so that we can cite the reference in this datasheet.

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