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Overview

  • Product name
    Anti-MLK4 antibody - N-terminal
    See all MLK4 primary antibodies
  • Description
    Rabbit polyclonal to MLK4 - N-terminal
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human MLK4 conjugated to KLH.

  • Positive control
    • 293 cell lysates transiently transfected with the MLK4 gene. Human colon carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab71510 is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 114 kDa.

Target

  • Function
    Activates the JUN N-terminal pathway.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
  • Information by UniProt
  • Database links
    • Alternative names
      • dJ862P8.3 antibody
      • KIAA1804 antibody
      • M3KL4_HUMAN antibody
      • Mitogen activated protein kinase kinase kinase antibody
      • Mitogen-activated protein kinase kinase kinase MLK4 antibody
      • Mixed lineage kinase 4 antibody
      • MLK 4 antibody
      • Mlk4 antibody
      • RP5 862P8.2 antibody
      see all

    Anti-MLK4 antibody - N-terminal images

    • Western blot - MLK4 antibody - N-terminal (ab71510)
      Western blot - MLK4 antibody - N-terminal (ab71510)
      All lanes : Anti-MLK4 antibody - N-terminal (ab71510) at 1/50 dilution

      Lane 1 : Nontransfected 293 cell lysate
      Lane 2 : 293 cell lysate transiently transfected with the MLK4 gene

      Lysates/proteins at 2 µg per lane.


      Predicted band size : 114 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 130 kDa,50 kDa,72 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLK4 antibody - N-terminal (ab71510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLK4 antibody - N-terminal (ab71510)
      ab71510 at 1/10 dilution, staining MLK4 in human colon carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

    References for Anti-MLK4 antibody - N-terminal (ab71510)

    ab71510 has not yet been referenced specifically in any publications.

    Publishing research using ab71510? Please let us know so that we can cite the reference in this datasheet.

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