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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein A purified
ab71510 is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/50 - 1/100. Predicted molecular weight: 114 kDa.
Activates the JUN N-terminal pathway.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. Contains 1 SH3 domain.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
Information by UniProt
Anti-MLK4 antibody - N-terminal images
Western blot - MLK4 antibody - N-terminal (ab71510)
All lanes :
Anti-MLK4 antibody - N-terminal (ab71510) at 1/50 dilution
Lane 1 :
Nontransfected 293 cell lysate
Lane 2 :
293 cell lysate transiently transfected with the MLK4 gene
Lysates/proteins at 2 µg per lane.
Predicted band size :
Observed band size :
105 kDa (
why is the actual band size different from the predicted?
Additional bands at :
130 kDa,50 kDa,72 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLK4 antibody - N-terminal (ab71510)
ab71510 at 1/10 dilution, staining MLK4 in human colon carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
References for Anti-MLK4 antibody - N-terminal (ab71510)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"