Overview

  • Product name
  • Description
    Rabbit polyclonal to MLLT1
  • Specificity
    This antibody recognizes MLLT1
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A region within synthetic peptide: SEDEASFKSE SAQSSPSNSS SSSDSSSDSD FEPSQNHSQG PLRSMVEDLQ, corresponding to internal sequence amino acids 361-410 of Human MLLT1

  • Positive control
    • Raji cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/12500.
WB Use a concentration of 1.4 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Capable of activating transcription from synthetic reporter genes in both lymphoid and myeloid cells.
  • Involvement in disease
    Note=A chromosomal aberration involving MLLT1 is associated with acute leukemias. Translocation t(11;19)(q23;p13.3) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similarities
    Contains 1 YEATS domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ENL antibody
    • ENL_HUMAN antibody
    • LTG19 antibody
    • MLLT1 antibody
    • Myeloid/lymphoid or mixed lineage leukemia trithorax (Drosophila) antibody
    • Protein ENL antibody
    • YEATS domain containing protein 1 antibody
    • YEATS domain-containing protein 1 antibody
    • YEATS1 antibody
    see all

Images

  • Anti-MLLT1 antibody (ab49052) at 1.4 µg/ml + Raji cell lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Dey SS  et al. Mutual Information Analysis Reveals Coevolving Residues in Tat That Compensate for Two Distinct Functions in HIV-1 Gene Expression. J Biol Chem 287:7945-55 (2012). WB ; Human . Read more (PubMed: 22253435) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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