Overview

  • Product nameAnti-MLN64 antibody
  • Description
    Rabbit polyclonal to MLN64
  • Tested applicationsSuitable for: ICC/IF, IHC-P, ICC, IP, Inhibition Assay, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Cow, Human
  • Immunogen

    Other Immunogen Type corresponding to MLN64. Recombinant MLN64 protein.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. By Western blot, this antibody detects an ~53 kDa protein representing MLN 64 from rat adrenal gland tissue extracts. This antibody also detects an ~25 kDa protein from rat adrenal gland which may correspond to degradation product.

Target

  • FunctionBinds and transports cholesterol. Promotes steroidogenesis in placenta and brain.
  • Sequence similaritiesContains 1 MENTAL domain.
    Contains 1 START domain.
  • Cellular localizationLate endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAB 1 antibody
    • CAB1 antibody
    • Es 64 antibody
    • Es64 antibody
    • FLJ41370 antibody
    • Metastatic lymph node gene 64 protein antibody
    • Metastatic lymph node protein 64 antibody
    • MLN 64 antibody
    • Mln64 antibody
    • Protein CAB 1 antibody
    • Protein CAB1 antibody
    • Protein MLN 64 antibody
    • Protein MLN64 antibody
    • StAR related lipid transfer protein 3 antibody
    • StAR-related lipid transfer protein 3 antibody
    • STAR3_HUMAN antibody
    • STARD 3 antibody
    • StARD3 antibody
    • START domain containing 3 antibody
    • START domain-containing protein 3 antibody
    • Steroidogenic acute regulatory protein related antibody
    see all

Anti-MLN64 antibody images

  • Anti-MLN64 antibody (ab3478) at 1 µg/ml + Lysates prepared from rat adrenal gland

    Predicted band size : 49 kDa
    Western blot of MLN64 on rat adrenal gland extract using ab3478.
  • ab3478 staining MLN64 in Human ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours 10 minutes at 4°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 20 minutes at 25°C. An undiluted HRP-conjugated monoclonal was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab3478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MLN64 antibody (ab3478)

This product has been referenced in:
  • Qiu Y  et al. Association analysis of ERBB2 amplicon genetic polymorphisms and STARD3 expression with risk of gastric cancer in the Chinese population. Gene 535:225-32 (2014). IHC-P ; Human . Read more (PubMed: 24291029) »
  • Barbero-Camps E  et al. Endoplasmic reticulum stress mediates amyloid ß neurotoxicity via mitochondrial cholesterol trafficking. Am J Pathol 184:2066-81 (2014). Mouse . Read more (PubMed: 24815354) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Ovarian cancer)
Specification Ovarian cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step BSA as blocking agent for 12 hour(s) and 10 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mrs. Wu Xin

Verified customer

Submitted Oct 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"