For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Other Immunogen Type corresponding to MLN64. Recombinant MLN64 protein.
Our Abpromise guarantee covers the use of ab3478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Inhibition Assay||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. By Western blot, this antibody detects an ~53 kDa protein representing MLN 64 from rat adrenal gland tissue extracts. This antibody also detects an ~25 kDa protein from rat adrenal gland which may correspond to degradation product.|
ab3478 staining MLN64 in Human ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours 10 minutes at 4°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 20 minutes at 25°C. An undiluted HRP-conjugated monoclonal was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"