Overview

  • Product nameAnti-MLX antibody
    See all MLX primary antibodies
  • Description
    Rabbit polyclonal to MLX
  • Specificityab65147 detects endogenous levels of total MLX protein.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human MLX

  • Positive control
    • LOVO cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab65147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
WB
  • Application notesELISA: 1/10000.
    ICC/IF: Use at a concentration of 1 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
    • Tissue specificityExpressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    • Cellular localizationCytoplasm. Found predominantly in the cytoplasm and Nucleus. Found predominantly in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHd13 antibody
      • BigMax protein antibody
      • Class D basic helix-loop-helix protein 13 antibody
      • MAD7 antibody
      • Max like bHLHZip protein antibody
      • Max like protein X antibody
      • Max-like bHLHZip protein antibody
      • Max-like protein X antibody
      • MLX antibody
      • MLX_HUMAN antibody
      • MLXIP antibody
      • MONDOA antibody
      • MXD7 antibody
      • Protein BigMax antibody
      • Protein Mlx antibody
      • TCFL4 antibody
      • transcription factor like 4 antibody
      • Transcription factor like protein 4 antibody
      • Transcription factor-like protein 4 antibody
      see all

    Anti-MLX antibody images

    • All lanes : Anti-MLX antibody (ab65147) at 1/500 dilution

      Lane 1 : LOVO cell extract
      Lane 2 : LOVO cell extract with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 33 kDa
      Observed band size : 33 kDa
    • ICC/IF image of ab65147 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65147, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-MLX antibody (ab65147)

    ab65147 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab65147.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"