Anti-MLX-interacting protein antibody (ab77294)

Overview

  • Product nameAnti-MLX-interacting protein antibody
    See all MLX-interacting protein primary antibodies
  • Description
    Rabbit polyclonal to MLX-interacting protein
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MLX-interacting protein.

    (Peptide available as ab90826.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human heart; Human liver; Human kidney, as well as the following whole cell lysates: HeLa; HepG2; HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab77294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • RelevanceMLX-interacting protein is a basic helix-loop-helix leucine zipper transcription factor of the Myc/Max/Mad superfamily. This protein binds to DNA as a heterdimer with MLX, resulting in transcription activation. MLX-interacting protein plays a role in transcriptional activation of glycolytic target genes and is involved in glucose-responsive gene regulation.
  • Cellular localizationCytoplasmic, Mitochondrial uter membrane and Nuclear. Note: Predominantly cytoplasmic but shuttles between cytoplasm and nucleus when associated with MLX. Also associates with the outer mitochondrial membrane and may shuttle between the outer mitochondrial membrane and the nucleus.
  • Database links
  • Alternative names
    • BHLHE36 antibody
    • Class E basic helix loop helix protein 36 antibody
    • MIR antibody
    • MLXIP antibody
    • MONDOA antibody
    • Transcriptional activator MondoA antibody
    see all

Anti-MLX-interacting protein antibody images

  • All lanes : Anti-MLX-interacting protein antibody (ab77294) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 101 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
  • ICC/IF image of ab77294 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77294, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

References for Anti-MLX-interacting protein antibody (ab77294)

ab77294 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - other (Nuclei and mitochindria isolated from mice livers)
Loading amount 20 µg
Specification Nuclei and mitochindria isolated from mice livers
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 08 2012

I am very pleased to hear you would like to accept our offer and test ab77294 on mouse samples. This code will give you: one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse WB and include this...

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