Anti-MLYCD antibody (ab95945)

Overview

  • Product nameAnti-MLYCD antibody
    See all MLYCD primary antibodies
  • Description
    Rabbit polyclonal to MLYCD
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 90-274 of MLYCD (Human)

  • Positive control
    • 293T; A431; H1299; HepG2 or Raji whole cell lysates HeLa cells (IF)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • FunctionCatalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids.
  • PathwayMetabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from malonyl-CoA: step 1/1.
  • Involvement in diseaseMalonyl-CoA decarboxylase deficiency (MLYCD deficiency) [MIM:248360]: Autosomal recessive disease characterized by abdominal pain, chronic constipation, episodic vomiting, metabolic acidosis and malonic aciduria. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Cellular localizationMitochondrion. Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCMC_HUMAN antibody
    • hMCD antibody
    • Malonyl CoA decarboxylase antibody
    • Malonyl CoA decarboxylase mitochondrial antibody
    • Malonyl coenzyme A decarboxylase antibody
    • Malonyl-CoA decarboxylase antibody
    • MCD antibody
    • MGC59795 antibody
    • mitochondrial antibody
    • Mlycd antibody
    see all

Anti-MLYCD antibody images

  • Anti-MLYCD antibody (ab95945) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 55 kDa
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using: (1) ab95945 at 1/200 dilution, (2) merged with DNA probe.
  • Immunohistochemical analysis of paraffin embedded H661 xenograft, using ab95945 at 1/500 dilution.

References for Anti-MLYCD antibody (ab95945)

ab95945 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (whole rat heart lysate and rat insulinoma cells)
Gel Running Conditions Reduced Denaturing (4-20% gradient)
Loading amount 50 µg
Specification whole rat heart lysate and rat insulinoma cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Oct 13 2015

I am very pleased to hear you would like to accept our offer and test ab95945 in pig. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for pig and include this code in the “Add...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Muscle)
Loading amount 20 µg
Specification Muscle
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"