Overview

  • Product name
    MM2 probe
  • Tested applications
    Suitable for: Cellular activitymore details
  • General notes

    MM2 probe (ab172913) is a cell-penetrating phosphorescence-based O2 sensing probes enable direct high-resolution 2D and 3D mapping and quantitation of (intra)cellular O2. MM2 probe (ab172913) and NanO2 probe (ab172912), offer a simple and convenient approach to the direct real-time live-cell imaging in-vitro, in-vivo, and for 3D cultures on standard fluorescent microscopes (wide-field, confocal or two-photon).

    Number of Assays: 10 - 100 assays
    Number of tests vary depending on platform used in the assay. The probe contains sufficient material for either 100 assays in a standard 96 well plate or 10 assays in an imaging mini dish 35mm.

    Probe Preparation and Cell Loading
    Probe is supplied as 100 µg aliquots in plastic vials.

    1. Add 100 µL of water to the vial and vortex for 30 seconds to produce 100X stock. Dilute stock with medium to working concentration 10 µg/mL.

    2. Add this medium with probe to the cells, incubate at 37°C in CO2 incubator for 3-24h.
    Normally cell loading is conducted in a volume of:

    • 100 µL per well for standard 96-well plates
    • 1 mL for imaging mini-dishes (whole dish) or 200 µL for glass insert only.

    3. Wash the cells 1-2 times with fresh medium. Add medium, usually same volume as above.

    4. Conduct optical measurements.

    Measurement Conditions

    • Excitation optima: One-photon - 390-405 nm; Two-photon - 750-770 nm
    • Emission optima: Reference dye – 430-450 nm; Phosphorescent dye – 640-670 nm

    Ensure temperature equilibration of samples during the measurements since the probe is sensitive to both O2 and T.

    Handling & Storage

    • Undiluted probe should be stored in the dark at +4°C.
    • Diluted probe should be used on the same day. Stock can be stored for 1-2 weeks at +4°C.
    • Protect probe and samples from ambient light. Short-term exposures are allowed. Exposure time to high-energy excitation light should be minimised.

Properties

  • Form
    Lyophilised:100 µg
  • Storage instructions
    Store at +4°C. Store In the Dark.
  • Concentration information loading...

Applications

Our Abpromise guarantee covers the use of ab172913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Cellular activity Use at an assay dependent concentration.

References

ab172913 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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