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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Constituent: PBS
Concentration information loading...
Purity Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 32 kDa).
Function May be involved in the binding and intracellular trafficking of cobalamin (vitamin B12).
Tissue specificity Widely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes.
Pathway Cofactor biosynthesis; adenosylcobalamin biosynthesis.
Involvement in disease Defects in MMACHC are the cause of methylmalonic aciduria and homocystinuria type cblC (MMACHC) [MIM:277400]. MMACHC is a disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Affected individuals may have developmental, hematologic, neurologic, metabolic, ophthalmologic, and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood.
Sequence similarities Belongs to the MMACHC family.
Information by UniProt
Anti-MMACHC antibody images
Western blot - MMACHC antibody (ab68678)
All lanes : Anti-MMACHC antibody (ab68678) at 1/500 dilution Lane 1 : MMACHC transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 25 µg per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution Predicted band size : 32 kDa Observed band size : 27 kDa ( why is the actual band size different from the predicted?)
References for Anti-MMACHC antibody (ab68678)
has not yet been referenced specifically in any publications.
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