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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
1/100 - 1/200.
May be involved in the binding and intracellular trafficking of cobalamin (vitamin B12).
Widely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes.
Cofactor biosynthesis; adenosylcobalamin biosynthesis.
Involvement in disease
Defects in MMACHC are the cause of methylmalonic aciduria and homocystinuria type cblC (MMACHC) [MIM:277400]. MMACHC is a disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Affected individuals may have developmental, hematologic, neurologic, metabolic, ophthalmologic, and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood.
Belongs to the MMACHC family.
Information by UniProt
Anti-MMACHC antibody images
Western blot - MMACHC antibody (ab96195)
All lanes : Anti-MMACHC antibody (ab96195) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : Raji whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 32 kDa
Immunocytochemistry/ Immunofluorescence - MMACHC antibody (ab96195)
ab96195, at 1/100 dilution, staining MMACHC in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
References for Anti-MMACHC antibody (ab96195)
This product has been referenced in:
et al. Interaction between methionine synthase isoforms and MMACHC: characterization in cblG-variant, cblG and cblC inherited causes of megaloblastic anaemia. Hum Mol Genet N/A:N/A (2013).
Read more (PubMed: 23825108) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"