Overview

  • Product nameAnti-MMACHC antibody
    See all MMACHC primary antibodies
  • Description
    Rabbit polyclonal to MMACHC
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a sequence within amino acids 1-188 of Human MMACHC (NP_056321).

  • Positive control
    • 293T and Raji whole cell lysates; HeLa cells; A431, H1299, HeLaS3 and HepG2 cell lines

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionMay be involved in the binding and intracellular trafficking of cobalamin (vitamin B12).
  • Tissue specificityWidely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes.
  • PathwayCofactor biosynthesis; adenosylcobalamin biosynthesis.
  • Involvement in diseaseDefects in MMACHC are the cause of methylmalonic aciduria and homocystinuria type cblC (MMACHC) [MIM:277400]. MMACHC is a disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Affected individuals may have developmental, hematologic, neurologic, metabolic, ophthalmologic, and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood.
  • Sequence similaritiesBelongs to the MMACHC family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1810037K07Rik antibody
    • BOS_3654 antibody
    • cblC antibody
    • DKFZp564I122 antibody
    • FLJ25671 antibody
    • Methylmalonic aciduria (cobalamin deficiency) cblC type with homocystinuria antibody
    • Methylmalonic aciduria and homocystinuria type C protein antibody
    • Methylmalonic aciduria and homocystinuria type C protein homolog antibody
    • MGC134307 antibody
    • MMAC_HUMAN antibody
    • MMACHC antibody
    • OTTHUMP00000009243 antibody
    • RP11 291L19.3 antibody
    • RP23-177C18.3 antibody
    see all

Anti-MMACHC antibody images

  • All lanes : Anti-MMACHC antibody (ab96195) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 32 kDa
  • ab96195, at 1/100 dilution, staining MMACHC in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

References for Anti-MMACHC antibody (ab96195)

This product has been referenced in:
  • Fofou-Caillierez MB  et al. Interaction between methionine synthase isoforms and MMACHC: characterization in cblG-variant, cblG and cblC inherited causes of megaloblastic anaemia. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23825108) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"