Anti-MMD2 antibody (ab72706)

Overview

  • Product nameAnti-MMD2 antibody
    See all MMD2 primary antibodies
  • Description
    Mouse polyclonal to MMD2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human MMD2 (NP_940685.2, 246 a.a)

  • Positive control
    • Human kidney tissue lysate, MMD2 transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 23, 35 kDa (predicted molecular weight: 31 , 29 kDa). (isoform 1 and isoform 2, respectively.)

Target

  • RelevanceMMD2 is a homologue of MMD and both proteins are members of the so far uncharacterized Hemolysin-III (Hly-III) family, and are atypical members of the PAQR family. PAQR10/MMD2, has recently (Góñez et al.Nov/Dec 2008) been found to be expressed in mouse pancreatic islet beta cells, and may have a role in the endocrine pancreas development and hyperplasia in pregnancy.
  • Cellular localizationGolgi apparatus membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • FLJ37205 antibody
    • MMD 2 antibody
    • Monocyte to macrophage differentiation associated 2 antibody
    • Monocyte to macrophage differentiation associated protein 2 antibody
    • Monocyte to macrophage differentiation factor 2 antibody
    • PAQR10 antibody
    • Progestin and adipoQ receptor family member 10 antibody
    • Progestin and adipoQ receptor family member X antibody
    see all

Anti-MMD2 antibody images

  • Anti-MMD2 antibody (ab72706) at 1/500 dilution + human kidney lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 31 , 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-MMD2 antibody (ab72706) at 1/500 dilution

    Lane 1 : MMD2 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 31 , 29 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

References for Anti-MMD2 antibody (ab72706)

ab72706 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (15P1)
Specification 15P1
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% saponin
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
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Abcam user community

Verified customer

Submitted Feb 28 2012

You are very welcome! Have a great day.

Yes, you can place the order (both this one and the one for the free primary) as usual through the distributor. The expiration date is four months after issuing the code (see below), however, in individual cases we may be able to make an excemption and...

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Thank you very much for your interest in ab72706. So far we have tested ab72706 only in Western blot with human samples. However, the sequence similarity between the human and the murine MMD2 is 87% and a cross-reactivity of this antibody is likely...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"