Overview

  • Product name
    MMP-3 Activity Assay Kit (Fluorometric)
    See all MMP3 kits
  • Detection method
    Fluorescent
  • Sample type
    Cell culture supernatant, Milk, Urine, Tissue Extracts, Cell culture media
  • Assay type
    Enzyme activity
  • Sensitivity
    < 0.05 mU/well
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Mammal
  • Product overview

    In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is < 50 µU.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab118972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • MMP-3 Mca Standard
  • MMP-3 Positive Control

Protocols

References

This product has been referenced in:
  • O'Callaghan J  et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). Read more (PubMed: 28158775) »
  • Pettersson F  et al. Genetic and pharmacologic inhibition of eIF4E reduces breast cancer cell migration, invasion, and metastasis. Cancer Res 75:1102-12 (2015). Read more (PubMed: 25608710) »

See all 2 Publications for this product

Customer reviews and Q&As

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Since ab118972 is an enzyme assay, we would not recommend EDTA treated plasma for it. The customer could try using heparin instead for plasma.

I hope this information is helpful to you. Please do not he...

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The FRET substrate we provide in this kit is very specific for MMP-3. Other MMPs should not be able to hydrolyze it, therefore there should not be any interference of that sort.

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EDTA is known to inhibit MMP-3 activity in general conditions. However, we have not encountered this problem specifically. It might depend on the concentration of EDTA used. Nevertheless, it is always better to u...

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The molecular weight of the Active MMP-3 is: 19.5 kDa.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us. Each vial of MMP-3 positive control contains 2.2 ug of the active protein that has been lyophilized. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or infor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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