MMP-3 (Matrix Metalloproteinase-3), also known as stromelysin-1, is involved in wound repair, atherosclerosis and tumors. ab112148 MMP-3 Activity Assay Kit uses a Green Fluorescence probe/fluorescence resonance energy transfer (FRET) peptide as the MMP-3 activity indicator. In the intact FRET peptide, the fluorescence of one half of the probe is quenched by the other part. Upon cleavage into two separate fragments by MMP-3, the green fluorescence is recovered.
ab112148 is designed to monitor the activity of an MMP-3 enzyme. The peptide sequence used in the kit is more selective for MMP-3 hydrolysis than other MMP enzymes. It can also be used to screen MMP-3 inhibitors when a purified MMP-3 enzyme is used. With excellent fluorescence quantum yield and longer wavelength, the probe shows less interference from autofluorescence of test compounds and cellular components and is much more sensitive than an EDANS/Dabcyl FRET substrate. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/525 nm. The perfect excitation of the green probe at 488 nm makes the assay readily readable with almost all the common fluorescence instruments equipped with Argon laser and FITC filter set.
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ab112148 should be stored Desiccated
|APMA, 4-Aminophenylmercuric Acetate||1 x 20µl|
|Assay Buffer||1 x 20ml|
|MMP3 Green Substrate||1 x 60µl|
Our Abpromise guarantee covers the use of ab112148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab112148 has not yet been referenced specifically in any publications.