MMP1 antibody [3A9.3] - Whole molecule (ab38923)

Product Name 

MMP1 antibody [3A9.3] - Whole molecule

Product type 

Primary antibodies

Description 

Mouse monoclonal [3A9.3] to MMP1 - Whole molecule

Immunogen 

Full length native protein (purified) of Human MMP1.

(Peptide available as ab39297.)

Reacts with 
(species key)

Hu

Specificity 

ab38923 binds to reduced and native MMP1, but does not cross react with the other MMP family members (MMP2, MMP3, MMP9, etc.). It reacts with the "pro" and active forms of MMP1.

We have a range of domain specific antibodies for this target. For a full list please see all MMP1 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: A recommended starting concentration for Western blots is 1:1000 when using colorometric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP-1 is well conserved, higher concentrations of antibody may be needed for non-human samples. Collagenase levels in quiescent cells and tissues is minimal, and stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of Collagenase produced. Detects a band of approximately 51, 53 kDa (predicted molecular weight: 54 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Cell media from neonatal human fibroblasts treated with TNF alpha.

Cellular localization 

Secreted

Research areas 

Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

MMP1, also known as interstitial collagenase, is the only enzyme that is able to initiate the breakdown of the interstitial collagens, types I, II, and III.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

3A9.3

Isotype 

IgG1

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody has been affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

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