• Product name
    Anti-MMP1 antibody [3A9.3]
    See all MMP1 primary antibodies
  • Description
    Mouse monoclonal [3A9.3] to MMP1
  • Specificity
    ab38923 binds to reduced and native MMP1, but does not cross react with the other MMP family members (MMP2, MMP3, MMP9, etc.). It reacts with the "pro" and active forms of MMP1.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) of Human MMP1.

    (Peptide available as ab39297.)

  • Positive control
    • Cell media from neonatal human fibroblasts treated with TNF alpha.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab38923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 51, 53 kDa (predicted molecular weight: 54 kDa). A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Collagenase levels in quiescent cells and tissues is minimal, and stimulation of protein concentration is often needed to visualize the bands.


  • Function
    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 27 kDa interstitial collagenase antibody
    • CLG antibody
    • CLGN antibody
    • collagenase, fibroblast antibody
    • collagenase, interstitial antibody
    • Fibroblast collagenase antibody
    • Interstitial collagenase antibody
    • Matrix metallopeptidase 1 (interstitial collagenase) antibody
    • Matrix metalloprotease 1 antibody
    • Matrix Metalloproteinase 1 antibody
    • Matrix metalloproteinase-1 antibody
    • MMP 1 antibody
    • MMP-1 antibody
    • MMP1 antibody
    • MMP1_HUMAN antibody
    • OTTHUMP00000045866 antibody
    see all

Anti-MMP1 antibody [3A9.3] images

  • All lanes : Anti-MMP1 antibody [3A9.3] (ab38923) at 1/1000 dilution

    Lane 1 : Human MMP 1
    Lane 2 : Cell media from neonatal human dermal fibroblasts treated with TNF alpha

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 51,53 kDa (why is the actual band size different from the predicted?)

References for Anti-MMP1 antibody [3A9.3] (ab38923)

This product has been referenced in:
  • Pozgan U  et al. Expression and activity profiling of selected cysteine cathepsins and matrix metalloproteinases in synovial fluids from patients with rheumatoid arthritis and osteoarthritis. Biol Chem 391:571-9 (2010). Read more (PubMed: 20180636) »

See 1 Publication for this product

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