Overview

  • Product nameAnti-MMP1 antibody [3B6]
    See all MMP1 primary antibodies
  • Description
    Mouse monoclonal [3B6] to MMP1
  • SpecificityThis monoclonal antibody binds to human MMP-1. ab2461 targets both the active form and the proenzyme. This antibody was tested for cross-reactivity with MMP-2, MMP-3, and MMP-9 and did not cross-react.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    CSSFGFPRTVKH

    , corresponding to Internal sequence amino acids 365-376 of Human MMP1.

  • Positive control
    • colon carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab2461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
IHC-P 1/2. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • FunctionCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 27 kDa interstitial collagenase antibody
    • CLG antibody
    • CLGN antibody
    • collagenase, fibroblast antibody
    • collagenase, interstitial antibody
    • Fibroblast collagenase antibody
    • Interstitial collagenase antibody
    • Matrix metallopeptidase 1 (interstitial collagenase) antibody
    • Matrix metalloprotease 1 antibody
    • Matrix Metalloproteinase 1 antibody
    • Matrix metalloproteinase-1 antibody
    • MMP 1 antibody
    • MMP-1 antibody
    • MMP1 antibody
    • MMP1_HUMAN antibody
    • OTTHUMP00000045866 antibody
    see all

References for Anti-MMP1 antibody [3B6] (ab2461)

This product has been referenced in:
  • Silva EJ  et al. S100A8/A9 regulates MMP-2 expression and invasion and migration by carcinoma cells. Int J Biochem Cell Biol 55:279-87 (2014). WB ; Human . Read more (PubMed: 25236491) »
  • Murray GI  et al. Matrix metalloproteinases and their inhibitors in gastric cancer. Gut 43:791-7 (1998). Read more (PubMed: 9824606) »

See all 3 Publications for this product

Product Wall

Thank you for bringing this to our attention. Can you please describe your samples, how you prepare them for loading into the gel, how much antibody you use and how long you incubate, and any other details you think may be relevant? Are you confident t...

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Thank you for you enquiry. Further to correspondence with the source of this antibody I can tell you that this antibody targets both the active form and the proenzyme. I have updated the antibody datasheet as a result. I hope this information he...

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Thank you for your enquiry. The active enzyme has a Mw of 43 KDa. Thus in the blot you wil find both the 54 KDa band of the proenzyme, and also the 43 KDa band. I hope this information helps. Please do not hesitate to contact us if you need anyt...

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You do not state in which application you plan to use the antibodies. However, you mention procedure, pH, treatment, etc. and the fact that you are a medical student, so I assume that you are going to working with IHC on paraffin embedded sections. ...

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Thank you for your enquiry. I would recommend using a dilution of 1:1000 and then optimizing based on your results. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"