Anti-MMP1 antibody (ab38929)
Key features and details
- Rabbit polyclonal to MMP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MMP1 antibody
See all MMP1 primary antibodies -
Description
Rabbit polyclonal to MMP1 -
Host species
Rabbit -
Specificity
This antibody binds to MMP1, but does not cross react with the other MMP family members (MMP2, MMP3, MMP9). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to MMP1. based on the hemopexin domain of the human sequence
(Peptide available asab41003) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab38929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/5000. Predicted molecular weight: 54 kDa. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection.
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Notes |
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WB
1/5000. Predicted molecular weight: 54 kDa. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection. |
Target
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Function
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsUndergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4312 Human
- Omim: 120353 Human
- SwissProt: P03956 Human
- Unigene: 83169 Human
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Alternative names
- 27 kDa interstitial collagenase antibody
- CLG antibody
- CLGN antibody
see all
Images
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All lanes : Anti-MMP1 antibody (ab38929)
Lane 1 : Human MMP1.
Lane 2 : Cell Media from human chondrosarcoma (no treatment).
Lane 3 : Cell Media from human chondrosarcoma (treated with TPA).
Predicted band size: 54 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (53)
ab38929 has been referenced in 53 publications.
- Hammash D et al. miR-623 Targets Metalloproteinase-1 and Attenuates Extravasation of Brain Metastatic Triple-Negative Breast Cancer Cells. Breast Cancer (Dove Med Press) 14:187-198 (2022). PubMed: 35936987
- Dai L et al. Comprehensive bioinformatic analysis of MMP1 in hepatocellular carcinoma and establishment of relevant prognostic model. Sci Rep 12:13639 (2022). PubMed: 35948625
- Varshavsky JR et al. Organophosphate Flame Retardants, Highly Fluorinated Chemicals, and Biomarkers of Placental Development and Disease During Mid-Gestation. Toxicol Sci 181:215-228 (2021). PubMed: 33677611
- Gong D et al. Overexpression of HOXA10 promotes the growth and metastasis of nasopharyngeal carcinoma. Exp Biol Med (Maywood) 246:2454-2462 (2021). PubMed: 34293937
- Chen K et al. Disrupting biological sensors of force promotes tissue regeneration in large organisms. Nat Commun 12:5256 (2021). PubMed: 34489407