MMP1 antibody - Hemopexin domain (ab38929)

Product Name 

MMP1 antibody - Hemopexin domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP1 - Hemopexin domain

Immunogen 

Synthetic peptide based on the hemopexin domain of the human sequence. Ab38929 binds to the hemopexin domain of MMP1.(Peptide available as ab41003.)

Reacts with 
(species key)

Hu, Hrs

Specificity 

This antibody binds to MMP1, but does not cross react with the other MMP family members (MMP2, MMP3, MMP9).

We have a range of domain specific antibodies for this target. For a full list please see all MMP1 antibodies

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent concentration (PMID 18434409).
IHC-P: 1/20.
WB: A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP-1 is well conserved, higher concentrations of antibody may be needed for non-human samples. Collagenase is produced at very low levels in normal, quiescent cells and tissues. Collagenase production can be induced by mitogens such as TPA or TNF-a in receptive cell types. Rodent species appear to lack MMP-1, instead producing MMP-13 (collagenase-3), although both enzymes have been reported in human tissues. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection. Predicted molecular weight: 54 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cardiovascular >> Atherosclerosis >> Thrombosis >> Endothelial mediators/regulators
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis.

MMP1, also known as interstitial collagenase, is the only enzyme that is able to initiate the breakdown of the interstitial collagens, types I, II, and III.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

See below for MMP1 antibody - Hemopexin domain images, references, products related to ab38929 and other tools.

MMP1 antibody - Hemopexin domain images:

References for MMP1 antibody - Hemopexin domain (ab38929)

  This product has been used in: (two most recent references)

Stojic J et al. Expression of matrix metalloproteinases MMP-1, MMP-11 and MMP-19 is correlated with the WHO-grading of human malignant gliomas. Neurosci Res 60:40-9 (2008). PubMed: 17980449

Yuan ZQ et al. Transcriptional changes induced by bovine papillomavirus type 1 in equine fibroblasts. J Virol 82:6481-91 (2008). ICC/IF; Horse. PubMed: 18434409
If you publish research using ab38929 please let us know so that we can cite the reference on this datasheet.

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