Overview

  • Product name
    Anti-MMP1 antibody, prediluted
    See all MMP1 primary antibodies
  • Description
    Rabbit polyclonal to MMP1, prediluted
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal).

  • Positive control
    • Placenta, bladder, Breast and ovarian carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab15473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: Ready to use for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
    • Sequence similarities
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain
      There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications
      Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 27 kDa interstitial collagenase antibody
      • CLG antibody
      • CLGN antibody
      • collagenase, fibroblast antibody
      • collagenase, interstitial antibody
      • Fibroblast collagenase antibody
      • Interstitial collagenase antibody
      • Matrix metallopeptidase 1 (interstitial collagenase) antibody
      • Matrix metalloprotease 1 antibody
      • Matrix Metalloproteinase 1 antibody
      • Matrix metalloproteinase-1 antibody
      • MMP 1 antibody
      • MMP-1 antibody
      • MMP1 antibody
      • MMP1_HUMAN antibody
      • OTTHUMP00000045866 antibody
      see all

    Images

    • ab15473 staining MMP1 in human placenta by Immunohistochemistry (FFPE-sections).

    References

    ab15473 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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